IBI Scientific IB45000 Semi-Dry Blotter Vertical Electrophoresis System

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IBI Scientific IB45000 Semi-Dry Blotter Vertical Features

• Comes complete with cathode plate cover (black), anode plate base (red), power cords, and operation manual

• Rugged construction for long work life in your lab

• Large active transfer area for greater lab productivity

• Low buffer use for reduced operation cost

• Low heat production results in less band distortion

About IBI Scientific IB45000 Semi-Dry Blotter Vertical

IBI's Semi-Dry Blotters are electrophoretic devices designed for fast, efficient, and economic transfer of protein, RNA, or DNA from polyacrylamide or agarose gels to a solid membrane support which is subsequently visualized by staining or other immunological methods. The IBI units are designed to produce a very uniform system for the transfer of proteins and nucleic acids. The very low buffer volume and minimal current use produces very little heat, thus preventing band distortion. Transfer can occur in less than an hour, depending on the molecular weight of the molecules to be transferred. Either single gels or multiple gel stacks can be transferred with the units. Multiple gel system reduces lab work time. Fast transfer times increase lab productivity. Standard power supplies can be used to minimize start-up costs. The IBI Semi-Dry Blotter consists of a specially designed and tested cathode plate cover and a graphite anode plate. A trans- unit stack is formed by the gel and the membrane being in con- tact, and sandwiched between filter papers soaked in buffer. An electric current is applied perpendicular to the gel and buffer saturated filter papers. The bands from the charged molecules of the gels migrate and bind to the membrane, and this is dependent upon molecular size and charge. Several stacks of trans-units can be run simultaneously. IB45000 is designed for multiple gel transfers, 2-D transfers and larger gels up to 24 x 30cm. The graphite plates serve as the electrodes, which dissipate potential heat build-up during the electrophoretic transfer. 42" Cathode electrode wire. 42" Anode electrode wire. 6 Trans-units recommended maximum capacity.