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BioVision CaspSCREEN Flow Cytometric Apoptosis Detection Kit, K200-25
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About BioVision CaspSCREEN Flow Cytometric Apoptosis
Measures Activated Caspases in intact cells by FACS Detection method- Flow cytometry, L-1 channel (Ex/Em = 488/530 nm).Species reactivity- MammalianKit size- Convenient sizes (25 and 100 assays)Applications- Detection of activated caspases in living cells. Activation of members of caspase-family proteases plays a key role in apoptosis. The CaspSCREENTM Flow Cytometric Apoptosis Detection Kit provides a convenient means for detecting activation of caspases by flow cytometry in living cells. The assay is based on the cleavage of (aspartyl)2-Rhodamine 110 (Dâ‚‚R), a reported substrate for members of caspase family proteases. The caspase substrate Dâ‚‚R is non-fluorescent, however, upon cleavage of the substrate by cellular caspases, the released rhodamine 110 gives rise to fluorescence that can be measured at excitation of 488 nm and emission of 530 nm.
