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ACCUZYME™ is recommended for all routine cloning applications. ACCUZYME™ is a proprietary proofreading enzyme that offers increased-fidelity and high PCR yield, even in demanding applications. ACCUZYME™ has an error-rate of 3.0 x 10,-6 and results in blunt-ended amplicons of up to 5 kb in length, making it ideal for use in cloning and site-directed mutagenesis.ACCUZYME™ is supplied with a buffering system that provides ideal conditions for most assays. Consequently, the cost and effort typically associated with optimizing assay performance is often eliminated. However, in circumstances where further optimization is required to improve PCR specificity and/or yield, ACCUZYME™ includes an additional vial of MgCl2.