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IBI Scientific IB47120 Maxi Fast-Ion Plasmid Sample Kit for 2 Preparations
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IBI Scientific IB47120 Maxi Fast-Ion Plasmid Sample Features
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Kit contains 2 Maxi plasmid columns, 25mL PM1 buffer, 25mL PM2 buffer, 25mL PM3 buffer, 25mL PEQ buffer, 65mL PW buffer, 25mL PEL buffer and added RNASE A (50mg/mL)
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For research use only, ultra-pure and large-volume plasmid preparation
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Sample size: 100mL of cultured bacterial cells for high-copy number plasmid and 250mL of cultured bacterial cells for low-copy number plasmid
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Format: ion-exchange column
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Operation: gravity flow
About IBI Scientific IB47120 Maxi Fast-Ion Plasmid Sample
The fast ion plasmid maxi kit uses pre-packed anion-exchange resin columns to purify plasmid DNA from 100-250 ml of cultured bacterial cells. Modified Alkaline Lysis method (1) and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow procedure, plasmid DNA in the crude lysate is bound to the column and the contaminants are removed with the PW Buffer. The purified plasmid DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting. The entire procedure can be completed in 2.5 hours without ultracentrifuges, HPLC or other toxic reagents and the purified plasmid DNA is suitable for transfection, sequencing reactions, ligation, PCR, and in-vitro transcription, microinjection, restriction enzyme digestion and gene gun. The quality of the fast ion plasmid maxi kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 100 mL overnight E. coli (DH5 alpha) culture, containing plasmid pBluescript (A600 greater than 2 U/ml). More than 400 microgram of plasmid DNA is quantified with a spectrophotometer. The purified plasmid (1 microgram) is used in EcoR I digestion, and checked by electrophoresis. Caution: During operation, always wear a lab coat, disposable gloves, and protective goggles. Binding capacity: 500 microgram /1mg. Purity: Equal to that obtained by 2xCsCl-gradient centrifugation. Operation time: 120 minutes. Applications: transfection, sequencing, in vitro transcription, microinjection, restriction digestion.
