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Tufpak is the original manufacturer of Autoclave bags to industry. Autoclaving (steam sterilization) is the most efficient and economical wet thermal disinfection process. Hazardous waste producers in research and clinical laboratories rely on this process to continually meet their obligations to Federal and state regulations. after exposure to wet steam heat and autoclave pressure to visually show bag has been processed. Autoclave bags differ from everyday use bags because of the temperature resistance they have to maintain, and the strength requirements imposed by Federal regulations. after exposure to wet steam heat and autoclave pressure to visually show bag has been processed.