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NH-BSRS - NMDG-HEPES Brain Slice Recovery Solution (1000 ml)
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About NH-BSRS - NMDG-HEPES Brain Slice Recovery Solution
NMDG-HEPES Brain Slice Recovery Solution (1000 ml) (NH-BSRS) is used for recovery of acute brain slices from rats and mice after the cutting procedure. Product information Ready to Use NH-BSRS solution autoclaved Instructions for use: Ready to use: For 1000 ml NH-BSRS Solution: Oxygenate 500 ml Base A for 10 min Add slowly 500 ml Base B Oxygenate NH-BSRS Solution during use pH 7.4 when oxygenated with 95% O2, 5% CO2 (Carbogene) Use immediately once opened. Store at 4 - 8 °C Ional composition of NH-BSRS Solution N-Methyl-D-Glucamine = 93 mM NaHCO3 = 30 mM Glucose = 25 mM Na L-Ascorbate = 5 mM MgSO4 = 10 mM NaH2PO4 = 1,25 mM KCl = 2,5 mM CaCl2 = 0,5 mM HEPES = 20 mM Thiourea = 2 mM Na Pyruvate = 3 mM
