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10x Tricine SDS Running Buffer is formulated for separation of proteins in their denatured state on tricine gels. Tricine gels are specifically designed for the resolution of low molecular weight proteins. In this buffer system, tricine substitutes glycine in the running buffer, resulting in more efficient stacking and destacking of low molecular weight proteins and higher resolution of smaller peptides.
Recommended buffers: Use with tricine sample buffer to obtain optimal separation with tricine SDS running buffer.
Composition: 1x buffer contains Tris base (100 mM), tricine (100 mM), SDS (0.1%), pH 8.3
Storage: 4oC to room temperature for 1 year