Millipore UFC700308 Regenerated Cellulose Centricon Plus 70 Centrifugal Filter Unit, 3kDa NMWL, 60mm Diameter x 121mm Length, 19 sq cm Membrane (Pack of 8)

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About Millipore UFC700308 Regenerated Cellulose Centricon

The centricon plus 70 centrifugal filter is a disposable, single-use device designed for rapid processing of aqueous biological solutions in volumes ranging from 15 to 70 mL. It is compatible with swinging bucket centrifuges only. The centricon plus 70 device can concentrate most 70 mL solutions down to 350�L in 15-40 minutes. Flow rate is affected by several parameters, including the membrane nominal molecular weight limit (NMWL), solute molecular weight and concentration, viscosity, centrifugal force, and temperature. For maximum recovery, select a device with a NMWL approximately three times lower than the molecular weight of your target molecule. Expect longer spin times for starting solutions with over 5% solids. Flow rates at 4� degree C are typically 1.5 times slower than those at 25 degree C. Viscous solutions, such as 50% glycerine, will take up to five times longer to concentrate than very dilute solutions. Prerinsing: Centricon Plus-70 membranes contain trace amounts of glycerine, used as a humectant. To remove glycerine, fill device with 70�mL of buffer solution or Milli-Q water and spin for 5�minutes. To remove all water and buffer, a recovery spin is required. Do not allow the membrane in the filter device to dry out once wet. If the device is not being used immediately after prerinsing, leave fluid on the membrane until the device is used. Desalting or Buffer Exchange: Concentrate a 70 mL sample to 350�L. Discard filtrate and reconstitute the sample back to 70 mL by adding the required volume of buffer. Concentrate again to 350�L. Repeat this process until the concentration of the contaminating solutes is reduced. Typically, one wash cycle will remove > 95% of the initial salt content; two cycles will remove > 99.9% of the salt.