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Operation: Sure the eyepiece and diapbram get ready then put a microscope slides on the stage.Beginning with the 4x Objective. Turn on the LED Illumination. Turn the focusing knob until a clear image is obtained (First you need to find the object image with the coarse focusing,then adjust the object image to the clearest with the fine focusing). When the desired view is obtained under the lowest power (4x), rotate the nosepiece to the next higher magnification (10x)(40x).To sum up using the LED Illumination and coarse & fine focusing make the object image clearest and sharpest. Package: Lab Microscope Prepared Slide Set(5pcs) Blank slides(5 pcs) Pliers Lens cleaning paper