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For 40 plasmid isolations and 5 gels; Isolation of Plasmid DNA 60 min, electrophoresis 45 min
Kit includes: instructions, various solutions and buffers, agarose powder, Flashblue stain
You need: electrophoresis apparatus and power supply, waterbath, balance, microcentrifuge, microwave or hot plate, automatic pipet with tips
Visualization (white light), misc. labware, 95-100 percent isopropanol, distilled or deionized water, ice
In this experiment, students isolate plasmid DNA without the use of toxic chemicals such as phenol or chloroform
Description Small-scale rapid isolation of plasmid DNA is a routine procedure used for screening and analysis of recombinant DNAs in cloning and subcloning experiments. In this experiment, students isolate plasmid DNA without the use of toxic chemicals such as phenol or chloroform. Kit includes: instructions, various solutions and buffers, agarose powder, FlashBlue Stain. You need: electrophoresis apparatus and power supply, waterbath, balance, microcentrifuge, microwave or hot plate, automatic pipet with tips, visualization (white light), misc. labware, 95-100% isopropanol, distilled or deionized water, ice.