EZ-10 Spin Column Total RNA Miniprep Kit, 50 Preparations

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About EZ-10 Spin Column Total RNA Miniprep Kit, 50

The EZ-10 Column Total RNA Mini-Prep Kit allows efficient purification of total RNA from various samples, total RNA is easily purified from animal or human cells and tissues using a simple spin format. This kit simplifies total RNA isolation by combining the stringency of guanidine -isothiocyanate lysis with the speed and purity of silica-based purification. Protocol Overview Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide optimal binding conditions. The lysate is then loaded onto the EZ-10 column with a silica membrane. RNA binds to the silica membrane, all protein and other components are removed in the flow-through. Remaining contaminants and salts are efficiently washed away. Purified RNA is eluted in RNase-free water has OD260/OD280 ratios of 1.9-2.1 (measured in 10 mM Tris-HCl, pH 7.5) and is ideal for use in most downstream application including Northern blotting, RT-PCR, Quantitative PCR, Poly (A) RNA selection and Array analysis. Contents ✔️ Buffer RLT - 25ml  ✔️Buffer RW - 30ml ✔️ Universal RPE Solution - 12ml ✔️ RNase-free Water - 5ml ✔️ EZ-10 Spin Column - 50 pc ✔️ 2ml Collection Tube - 50 pc