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About 2xqPCR Master Mix (No ROX), 5x1ml
2xqPCR Master Mix (No ROX) is a convenient premixed 2x concentrated solution for SYBR Green I-based qPCR reagent. It contains PowerPCRTM Hot Start Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent dyes and Mg2+. It can be applied to the target sequence detection of genomic DNA and cDNA. The SYBR Green I dye binds to double-stranded (ds) DNA without using sequence-specific probes. The chemically modified PowerPCRTM Hot Start Taq DNA polymerase is inactive at room temperature which effectively avoids the nonspecific amplification caused by primer dimers or the nonspecific binding of prime and template. The enzyme should be activated by incubation at 95°C for 10 minutes. The unique PCR buffer components and the hot start enzyme ensure PCR specificity and sensitivity. It has a higher sensitivity and a wider linear range which can exactly quantitate the target gene or the low copy template.
