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About LDS Sample Buffer [4X] 250 Ml
The LDS Sample Buffer [4X] contains 988 mM Tris, 2.04 mM EDTA, 8 % LDS (Lithium dodecyl sulfate), 40 % Glycerol, 0.88 % Commassie Brilliant Blue G250, 0.7 mM Phenol red and doesn't contain any reducing agent. Use with a protein reducing agent such DTT, TCEP (10 mM; use freshly prepared) or Beta meracptoethanol (2%) to reduce disulfide bonds in the protein sample.
